Ordering, Design, Manufacturing and Assembly Instructions for Cap:¶

  • Part contains a Two Custom Coils which I estimated using Trial and error.
    • The Two Coils Are 180 Degrees apart. The Screenshot should give you the dimensions of the first coil.
    • I then moved these coils and fillet them before fusing them with the main body in the Model.
    • The F3D File should have the complete design history.
    • I have also provided Intermediate Versions of the STEP file to help if you don't have Autodesk Fusion.
  • You can also modify the design to make a two port version.
    • One of the Sketches in the F3D file should allow you to make the two port version.
  • I ordered these parts from RapidDirect
    • CNC Machining / Aluminum 6061-T6 / As machined (Ra1.6)
    • I orderd both: As Machined and Anodized parts.
    • I recommend anodizing since you can autoclave the parts if necessary.
      • Anodizing Type II (Black, Glossy)
    • General tolerance: ISO2768M
    • Tightest tolerance: ISO2768F
    • Threads and tapped holes: Standard threads
      • In the notes please do mention: 1/4-28 UNF 1B(Loose Fit) is needed.
      • I've also included a Drawing document but did not mention details of the Custom Thread. Please do include the Dimensions of the Custom Coils in the Notes
    • You can also send them the intermediate files and the Screenshot with the Dimensions of the Coils to RapidDirect.
  • Cost
    • I ordered 60 Caps and the total came to about 11 USD per cap including shipping in May 2024.
    • Commercial Versions of these reservoirs cost about 120 USD or more. I recommend having them made with these CAD files to save money.
  • Finished Part Videos. You can watch videos of the Machined Finished Parts here:
    • Anodized Version
    • As-Machined Version
    • Both Parts Work Equally Well. But I recommend getting the Anodized version.
    • 30 Anodized Caps should be enough for one ChronoSeq device.
  • Adding Gasket/O-Ring
    • O-Ring Gasket Was Ordered from Primal23Industrial
    • Place this O-Ring at the Bottom of the Cap so it seals the Gap between the 50ml Falcon Tube and the CNC Machined Cap.
    • You can also order from Amazon
      • E12534116-100 1 1/4 OD X 3/4 ID X 1/16 Thickness Neoprene Rubber Washers - Endeavor Series Select Pack Quantity: 100 Pack. Cost about 20$.
  • Additional Parts:
    • To Block one of the Ports you can use a Delrin Plug.
    • You can Connect Pneumatic Tubing Using a Barb.
    • You can connect 1/16" OD tubing to the CAP using a Nut and Ferrule.
  • Leak Test Video:
    • In this Video the Fully Assembled Part was Submerged in Water and there were no leaks when pressurized to 2 Bar.
  • Optional Additive Manufacturing Version:
    • You can also print these parts using an FDM Printer but removing supports can be tricky.
      • You can use a toolkit like this to help with support removal.
    • SLA (Resin) Prints with a 50μm layer height is recommended.
    • In addition you will need to add another O-Ring in the 1/4-28 UNF Ports to prevent gas leaks, especially for the FDM Printed Version.
      • Oil-Resistant Soft Buna-N O-Rings, 1/16 Fractional Width, Dash Number 004, Packs of 100
    • The Ferrule should be in contact with the additional O-Ring to Prevent Leaks for the FDM Version.
  • Tolerance Compensation for FDM and SLA Prints
    • Tolerance compensation was needed for the FDM Version of the CAP. I used a XY Tolerance Compensation of 0.15mm
    • The 3MF File and was printed on a Bambu Lab X1 Carbon.
      • You can load the 3MF File into the Orca Slicer directly to inherit the print settings.
    • For the Bambu Lab X1 Carbon we had:
      • 0.4mm Nozzle
      • Textured PEI Plate
    • For the Resin Prints Tolerance compensation might also be needed. But I haven't tried it. Instead I:
      • Used a Tap and Tap Wrench to shave off excess plastic from the print.
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