ChronoSeq Links
Explore the key resources and documentation for the ChronoSeq project
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Main Hardware Pages
Essential hardware documentation
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Data Processing & Analysis
Tools and repositories for data analysis
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Community
Connect with ChronoSeq users and get support
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Hardware Assembly
Detailed assembly instructions for ChronoSeq hardware components
- Instructions For Assembling Sensirion Flow Sensor
- Instructions For Assembling Valve Controllerv2
- Instructions For Assembling Vortex Relay Controller
- Instructions For Assembling Xy Robot And Ice Boxes
- Instructions For Assembling Xyz Robot
- Instructions For Device Assembly And Setup
- Instructions For Setting Coordinates For Xyz Robot
- Instructions For Setting Up Valves Tubing And Reservoirs
⚠️ Deprecated Instructions
Note: These instructions are deprecated and may not work with current versions.
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Protocols
Laboratory protocols and procedures
- Protocol And Software For Running Chronoseq Device
- Protocol For Aquapel Treatment Of Microfluidic Chips
- Protocol For Bonding Pdms Chips To Glass
- Protocol For Bulk Rna Seq Library Prep
- Protocol For Making Mold Using Su8 Photolithography
- Protocol For Pdms Molding
- Protocol For Preparing 3.33X Lysis Buffer Stock
- Protocol For Preparing Cells Additionalfiltration
- Protocol For Preparing Chronoseq Dropseq Beads Stored In Ethanol
- Protocol For Qpcr Checking Of K562 Costimulation Using Chronoseq Device
- Protocol For Qpcr Validation Of Chronoseq Results
- Protocol For Recycling Beads
- Protocol For Single Cell Scale Up Chronoseqv5 Dropseq Bead Modification
- Protocol For Tagmentation With Kapa Pcr
- Protocol Library Preparation For Dropseq Chronoseq Beads
⚠️ Deprecated Protocols
Note: These protocols are deprecated and may not work with current versions.
- Protocol And Software For Running Chronoseq Device-Originaldevice
- Protocol For Chronoseqv3 And Dropseq Bead Modification 100Um Or 10Um
- Protocol For Chronoseqv3 And Dropseq Bead Modification 1Mm
- Protocol For Chronoseqv4 Bead Modification For Bulk Time Series
- Protocol For Exo1 Predigestion For Chronoseqv2 And Dropseq Beads
- Protocol For Preparing Cells
- Protocol For Preparing Cells Experimentalusingbsa
- Protocol For Qpcr Checking Of K562 Costimulation
- Protocol For Single Cell Scale Up Chronoseqv4 Dropseq Bead Modification
- Protocol Library Preparation For Dropseq Chronoseq Beads Previous Version
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Other Resources
CAD files, notebooks, and validation files
Cad Files > 3 Port Reservoir
Cad Files > Xy Robot > Attribution
Cad Files > Xyz Robot > Attribution
Qpcr Validation Files
Qpcr Validation Files > K562 Costimulation 2 Samples